Which of the following would not interfere with hemoglobin determination by the cyanmethemoglobin method?

The cyanmethemoglobin method is a widely used clinical technique for measuring hemoglobin levels in a blood sample. It relies on the conversion of hemoglobin to cyanmethemoglobin in a solution, which is then measured colorimetrically. Factors that might interfere with the accuracy of this determination typically include abnormalities in hemoglobin structure or other components that can affect the measurement's reliability.

Fetal hemoglobin (HbF) does not interfere with the cyanmethemoglobin method because it also converts to cyanmethemoglobin just like adult hemoglobin. While fetal hemoglobin has a different structure than adult hemoglobin (HbA), it is still effectively quantifiable using this method, allowing for accurate measurement in samples that contain it.

In contrast, increased lipids and elevated white blood cell counts may cause turbidity in the sample, which can affect the optical density readings required for accurate hemoglobin measurement. Lyse-resistant red blood cells could also lead to inaccurate results if they do not properly lyse, as incomplete lysis can inhibit the full conversion of hemoglobin to cyanmethemoglobin. Thus, fetal hemoglobin does not partake in the same interferences, making it the exception in this case.